Extrachromosomal DNA in Brain Metastases

Mapping the dynamics of ecDNA evolution during metastasis to the brain

A focused resource for cohort overview, ecDNA structures, and FISH validation.

Manuscript under review.

0tumor specimens, including 111 brain metastases and matched primary tumors.

0primary cancer types represented

0unique ecDNAs identified

0BrM patients with detected ecDNA

ecDNA cohortecDNA was recurrently detected across multiple primary tumor types.

Oncogenic cargoRecurrent amplified genes include ERBB2, MIEN1, JUP, FGFR2, CDK12, and SPAG9.

Amplification strengthecDNA-associated oncogenes showed higher copy-number levels than non-ecDNA focal amplicons.

Paired evolutionBrain metastases showed retained or newly gained ecDNA-associated oncogenic events.

Clinical associationecDNA positivity was linked to earlier brain metastasis and poorer survival.